A simple, easy-to-use, diagnostic test to identify and monitor accumulation of subconcussive head impacts has been developed by the researchers Texas Christian University and the University of Wisconsin-La Crosse. In American football, athletes are regularly subjected to subconcussive head impacts - hits to the head that are not severe enough to cause a concussion or even produce a clinical symptom. Nevertheless, the accumulation of subconcussive head impacts over the course of a football season has been linked to neurophysiological and neuropsychological changes in athletes, and the accumulation of subconcussive head impacts over a long career has been suggested as a cause of severe neurodegenerative diseases such as Alzheimer's disease (lat. Morbus Alzheimerianus) and chronic traumatic encephalopathy (CTE).

The team developed a schedule of blood (lat. Sanguis) sampling that is cost-effective and could fit into the busy schedule of an academic football program. To test the feasibility of this method, they evaluated the usefulness of two well-known biomarkers of head trauma and more severe traumatic brain injury: the tau protein and neurofilament light polypeptide (NF-L).

The researchers obtained blood samples from thirty-five NCAA football players on seven different days, beginning before fall camp commenced and ending three weeks after the competitive football season had finished. The researchers wanted to examine fluctuations in plasma concentrations of tau and serum concentrations of NF-L over the course of the entire football season, during which the number and magnitude of head impacts varied. Those athletes who sustained a concussion were not further examined. The timing of blood sampling was coordinated with the convenience of players and coaches. During the competitive period, this was usually between 36 and 72 hours after a game or practice session.

Analyses of the blood samples showed that tau concentrations decreased over the course of the competitive season, compared to preseason values, in both starters and nonstarters. Concentrations did not increase to preseason values until after the competitive season was over. Statistical analysis showed that differences in tau concentrations between the two groups of athletes (starters and nonstarters) were not great enough to differentiate between starters and nonstarters. In this study, tau concentrations were not useful in determining injury due to repetitive subconcussive head impacts.

Concentrations of NF-L increased in both athlete groups during the competitive season, compared to preseason values. In starters, these increases were significantly higher than preseason values at many time points; in nonstarters, the increases in NF-L concentrations never reached statistical significance. Statistical analysis showed that during periods of repetitive head impacts, differences in NF-L concentrations demonstrated fair to modest accuracy for differentiating between starters and nonstarters.

The researchers conclude that, in this preliminary study, serum NF-L shows some promise as a sensible and reliable biomarker of brain injury due to repetitive subconcussive head impacts. However, they point out that this study is preliminary and that additional, larger studies should be performed to verify this. They believe that the knowledge gained by studies of biomarkers will lay the groundwork for the eventual development of clinical tools to help reduce the deleterious effects of repetitive subconcussive impacts. Given recent findings indicating a potential link between repetitive subconcussive impacts and the development of CTE, efforts to determine the effect of subconcussive impacts throughout an athlete's career may prove useful, especially if those efforts are feasible and cost-effective.