NanoSurface Biomedical Inc. is working on biomarkers for developing nanopattern substrates, printed at the bottom of the sterile cell culture dishes, which provide a topographical and mechanical niche for cells to grow as if they are in vivo. By mimicking the extracellular environment, this unique Anisotropically NanoFabricated Substratum (ANFS) technology promotes the in vivo-like maturation and alignment of in vitro cell cultures. The NanoSurface Biomedical's nanopatterned cultureware has been already validated on various cell types such as cardiomyocytes, muscle cells, endothelial cells, iPS cells, mesenchymal stem cells, fibroblasts (to name a few) in vitro cell cultures. Currently, the company is developing synthetic human heart tissue for drug trials that will vastly reduce the time and cost of new drugs to market. NanoSurface Biomedical was selected from 40 companies to win $125,000 of funding at May’s Seattle Angel Conference.

NanoSurface Cultureware structures cultured cells and tissues to improve physiological relevance. Shortly after plating, cells cultured in NanoSurface Cultureware exhibit enhanced structural and phenotypic development as compared to cells cultured in conventional culture dishes. NanoSurface topography induces cytoskeletal reorganization and cellular alignment. NanoSurface Cultureware is available in familiar standard formats, featuring No. 1.5 glass-bottom wells for high-quality imaging. NanoSurface dishes promote the structural and phenotypic development of many cell types. Without biomimetic surface topography, cardiomyocytes exhibit random orientation, disorganized contraction patterns, and immature functional phenotypes on conventional culture surface. On NanoSurface dishes, cardiomyocytes align to the surface topography, elongate in the direction of the nanopattern, and exhibit uniform anisotropic contraction patterns, with enhanced contractile force and conduction velocity. NanoSurface nanopatterned culture surfaces provide a cellular microenvironment that mimics the aligned architecture of the native extracellular matrix, promoting cellular structural and functional development.

NanoSurface Cytostretcher is cell stretching instruments for biomimetic experiments. NanoSurface nanopatterned culture surfaces provide a cellular microenvironment that mimics the aligned architecture of the native extracellular matrix, promoting cellular structural and functional development. The NanoSurface Cytostretcher integrates flexible NanoSurface culture chambers with the ability to stimulate cultures with cyclic mechanical stretch. Versatile software enables programming of a wide array of stretch protocols for tremendous investigative flexibility.

The NanoSurface Operational Mechanics Interface - NaOMI - enables complete control of stretching parameters and cyclic strain protocols for maximum investigative flexibility. Compatible with both Windows and Mac OS, NaOMI software provides a straighforward and powerful user interface for automated operation of the NanoSurface Cytostretcher. USB connectivity allows for easy operation. Software logging automatically records experimental details. Users can program elongation length, holding time, and stretching speed independently for each stretch cycle.