A gene therapy that blocks this process, preventing axon destruction in mice and suggesting a therapeutic strategy that could help prevent the loss of peripheral nerves in multiple conditions has been developed by the scientists at Washington University School of Medicine in St. Louis (WUSTL). The strategy could help prevent peripheral neuropathy, a disease that currently affects about 20 million people in the United States. Peripheral neuropathy can result from chemotherapy for cancer treatment or poorly controlled diabetes, and it causes persistent pain, numbness, burning, itching and muscle weakness.
Many peripheral neuropathies are caused by the breakdown of nerve fibers, but we currently don’t have therapies that can directly block this process. When an axon is injured, whether cut or crushed by injury or damaged by drugs, a protein called SARM1 becomes active. In healthy nerves, this protein is switched off. Past studies by this research team have shown that activated SARM1 triggers axons to self-destruct, kicking off a chain of events that quickly consumes all of a nerve cell’s energy supply. The axons of such cells break into pieces.
In this study, scientists used a virus - one that can’t cause the disease - to deliver into cells a mutated version of the SARM1 protein that blocks axon destruction. This mutated SARM1 prevents the characteristic rapid energy loss and subsequent destruction of axons, even in the most extreme form of injury - a complete severing of the axon.
With the viral gene therapy, the researchers delivered a mutated form of SARM1 that is not only inactive itself but also blocks normal SARM1 proteins that have become activated in mice with nerve injuries. For a long time, viral gene therapy was a pipe dream, but there are now a number of ongoing clinical trials in other disorders that suggest the researchers are on a promising track.
For example, similar viral gene therapy is now in clinical trials for a genetic disorder called Duchenne muscular dystrophy. In that case, a different protein is delivered to address muscle loss, but the virus is the same. In theory, it could be possible to change the viral packaging to direct the viruses to deliver their gene payload to different types of cells - sensory neurons for peripheral neuropathy or motor neurons for ALS, for example.
This has the potential to be transformative because it cuts across so many diseases. Rather than addressing a single disease, it is potentially a treatment for a disease process that is shared among many different neurodegenerative disorders. In addition to this viral gene therapy, the researchers are studying other possible ways to block SARM1, including small molecules for drug development.